GITR:GITRL TR-FRET Assay Kit

• Description:

  The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against GITR:GITRL? Check out ourImmunotherapy Biochemical Screening Services.

• UniProt:

  Q7TS55

• Format:

  CatalogNumberComponentAmountStorage71256GITR, biotinylated15 µg-80°CAvoidfreeze/thawcycles!71190GITRL-His2 µg-80°CAnti-His Tb Donor2 x 10 µl-20°CDye-labeled Acceptor2 x 10 µl-20°C3x GITR TR FRET Assay Buffer4 ml-20°CWhite, non-binding, low volume, 384-well microtiter plate1RoomTemp.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.

• Notes:

  1. Lechner, M.G., et al. Immunotherapy. 2011 Nov; 3 (11) : 1317–1340.2. Ko, K., et al., J. Exp. Med. 2005; 202 (7) : 885-891.