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GITR:GITRL TR-FRET Assay Kit

CAT: 0421-79054Size: 384 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-79054Size:384 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The GITR:GITRL TR-FRET Assay is designed to measure the inhibition of GITR binding to GITRL in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing biotinylated GITR, GITRL, anti-His Tb donor, dye-labeled acceptor, and an inhibitor is incubated for two hours. Then, the fluorescence intensity is measured using a fluorescence reader.Need us to run inhibitor screens or profile your compounds against GITR:GITRL? Check out ourImmunotherapy Biochemical Screening Services.
UniProt
Q7TS55
Format
CatalogNumberComponentAmountStorage71256GITR, biotinylated15 µg-80°CAvoidfreeze/thawcycles!71190GITRL-His2 µg-80°CAnti-His Tb Donor2 x 10 µl-20°CDye-labeled Acceptor2 x 10 µl-20°C3x GITR TR FRET Assay Buffer4 ml-20°CWhite, non-binding, low volume, 384-well microtiter plate1RoomTemp.
Shipping Conditions
-80°C
Storage Conditions
Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Notes
1. Lechner, M.G., et al. Immunotherapy. 2011 Nov; 3 (11) : 1317–1340.2. Ko, K., et al., J. Exp. Med. 2005; 202 (7) : 885-891.

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