SARS-CoV-1 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit

• Background:

  Severe acute respiratory syndrome (SARS) is a viral respiratory illness caused by a corona virus called SARS-CoV-1. It was first reported in February 2013 in Asia and the epidemic affected ~ 26 countries and resulted in more than 8000 deaths. Like SARS-CoV-2, SARS-CoV-1 Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-1 and ACE2 may offer some protection against the viral infection.

• Description:

  The SARS-CoV-1 Spike Trimer (S1+S2) :ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors of the interaction of ACE2 ((angiotensin-converting enzyme 2) with the Spike Trimer (S1+S2) protein. This kit comes in a convenient 96-well format, with purified SARS-CoV-1 Spike Trimer (S1+S2) and ACE2-Biotin (amino acids 18-740) proteins, streptavidin-HRP, colorimetric HRP substrate, and assay buffer for 100 binding reactions. The SARS-CoV-1 Spike Trimer (S1+S2) protein included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-1/ACE2 interaction.Figure 1. Assay Kit schematic.First, SARS-CoV-1 Spike Trimer (S1+S2) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, Colorimetric HRP substrate is added to produce color that can be measured using a UV/Vis spectrophotometer microplate reader. The colorimetric signal is proportional to the efficacy of the binding of Spike Trimer (S1+S2) to ACE2.Need us to run an inhibitor screening or profiling? Check out ourCoronavirus Biochemical Screening Services.

• UniProt:

  P59594

• Applications:

  Screening inhibitors of ACE2 binding to SARS-CoV-1 Spike Trimer (S1+S2) .

• Format:

  Catalog #NameAmountStorage100789Spike Trimer (S1 + S2), His-Tag (SARS-CoV-1) *5 µg-80°C100665ACE2, His-Avi-Tag, Biotin-Labeled*5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79651Colorimetric HRP Substrate10 ml+4°C79964Transparent 96-well microplate1Room Temp*The initial concentration of the proteins is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  Up to 6 months from date of receipt, when stored as recommended.

• Contraindications:

  This assay kit is compatible with up to 1% final DMSO concentration.