LDLR, FLAG-Avi-Tag (Mouse) Recombinant

• Background:

  The low density lipoprotein receptor (LDLR protein) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. In case of HIV-1 infection, it functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the LDLR protein, inducing LDLR degradation.

• Description:

  Mouse low density lipoprotein receptor (LDLR), also known as FH, FHC, and LDLCQ2, GenBank Accession No. NM_010700, a.a. 22-790, with C-terminal Flag-tag, MW=87 kDa87, expressed in a HEK293 cell expression system. *Note: LDLR is heavily glycosylated, resulting in higher molecular weight.

• Tag:

  C-terminal FLAG-Avi-tag

• Applications:

  Useful for studying protein binding and screening small molecules for drug discovery.

• Purity:

  ≥ 88%

• Shipping Conditions:

  -80°C

• Storage Conditions:

  At least 6 months at –80°C. Avoid freeze thaw cycles. Storing diluted protein is not recommended, if necessary, use a carrier protein (BSA 0.1 – 0.5%) .

• Notes:

  * LDLR is heavily glycosylated, resulting in higher molecular weight. The two bands shown correspond to differing states of glycosylation.

• Calculated Molecular Weight:

  87 kDa

• Formulation:

  40 mM Tris HCl pH 8.0,110 mM NaCl, 2.2 mM KCl, and 20% glycerol.