SingleFlowEx HLA-DR Alexa Fluor® 700

SingleFlowEx HLA-DR Alexa Fluor® 700

• Description: SingleFlowEx is fluorochrome-labeled monoclonal antibody single reagent for use in laboratory-developed multicolor flow cytometry tests in order to examine human clinical samples. SingleFlowEx can be used in various multicolor combinations. Laboratory shall establish the SingleFlowEx performance characteristics in conjunction with other reagents in normal and pathological samples.
• Certification: RUO, GMP
• Clone: L243
• Validated Applications: FC
• Concentration: 0.060 mg/mL
• Format: Alexa Fluor® 700
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Components: 1 vial of monoclonal antibody reagent. The content (1 mL) is sufficient for min. 100 Tests.
• References & Citations: *Coral S, Pucillo C, Leonardi A, Fonsatti E, Altomonte M, Maio M: Triggering of HLA-DR antigens differentially modulates tumor necrosis factor alpha release by B cells at distinct stage of maturation. Cell Growth Differ. 1997 May8(5):581-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/9149909 ,*Muczynski KA, Ekle DM, Coder DM, Anderson SK: Normal human kidney HLA-DR-expressing renal microvascular endothelial cells: characterization, isolation, and regulation of MHC class II expression. J Am Soc Nephrol. 2003 May14(5):1336-48., URL: http://www.ncbi.nlm.nih.gov/pubmed/12707403 ,*Cantin R, Martin G, Tremblay MJ: A novel virus capture assay reveals a differential acquisition of host HLA-DR by clinical isolates of human immunodeficiency virus type 1 expanded in primary human cells depending on the nature of producing cells and the donor source. J Gen Virol. 2001 Dec82(Pt 12):2979-87. , URL: http://www.ncbi.nlm.nih.gov/pubmed/11714974 ,*Swiatek-de Lange M, Rist W, Stahl HF, Weith A, Lenter MC: Comment on "MHC class II expression identifies functionally distinct human regulatory T cells". J Immunol. 2008 Mar 15180(6):3625 author reply 3626., URL: http://www.ncbi.nlm.nih.gov/pubmed?term=comment%20on%20mhc%20class%20ii%20expression%20identifies ,*De Gassart A, Camosseto V, Thibodeau J, Ceppi M, Catalan N, Pierre P, Gatti E: MHC class II stabilization at the surface of human dendritic cells is the result of maturation-dependent MARCH I down-regulation. Proc Natl Acad Sci U S A. 2008 Mar 4105(9):3491-6., URL: https://pubmed.ncbi.nlm.nih.gov/18305173/,*Ivanov A, Beers SA, Walshe CA, Honeychurch J, Alduaij W, Cox KL, Potter KN, Murray S, Chan CH, Klymenko T, Erenpreisa J, Glennie MJ, Illidge TM, Cragg MS: Monoclonal antibodies directed to CD20 and HLA-DR can elicit homotypic adhesion followed by lysosome-mediated cell death in human lymphoma and leukemia cells. J Clin Invest. 2009 Aug119(8):2143-59., URL: http://www.ncbi.nlm.nih.gov/pubmed/19620786 ,*Kalka-Moll WM, Tzianabos AO, Bryant PW, Niemeyer M, Ploegh HL, Kasper DL: Zwitterionic polysaccharides stimulate T cells by MHC class II-dependent interactions. J Immunol. 2002 Dec 1169(11):6149-53., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tzianabos+ao+bryant+pw ,*Bouillon M, El Fakhry Y, Girouard J, Khalil H, Thibodeau J, Mourad W: Lipid raft-dependent and -independent signaling through HLA-DR molecules. J Biol Chem. 2003 Feb 28278(9):7099-107., URL: http://www.ncbi.nlm.nih.gov/pubmed/12499388,*Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug20(8):1174-1178. doi: 10.1038/s41592-023-01846-7., URL: https://pubmed.ncbi.nlm.nih.gov/37468619/,*Use of this product has not been published., URL: ,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.