Desmin Antibody

• Description :

  Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain -helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles.Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.

• Specifications :

  Immunohistochemistry (FFPE) : 2-4 µg/mL

• UniProt :

  P17661

• Host :

  Mouse

• Reactivity :

  Human, Dog, Cat, Horse, Cow

• Immunogen :

  Recombinant full-length human desmin protein was used as the immunogen for the Desmin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  DES/3255

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the Desmin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Desmin antibody is available for research use only.

• Storage Conditions :

  Aliquot the Desmin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the Desmin antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasm

• Image Legend :

  IHC staining of FFPE human cardiac muscle tissue with Desmin antibody (clone DES/3255) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.