NOC4L Antibody

• Description:

  Treg activation is critical for maintaining self-tolerance, but the translational control of this process is still poorly understood. Zhu et al. report a conserved ribosome biogenesis factor, Noc4L, that regulates mRNAs related to Treg and Tconv activation but does not affect global protein translation. Noc4L regulates translation of mRNAs related to Treg activation. Noc4L plays critical roles in activation of Tregs and Tconvs. Noc4L-mediated ribosome biogenesis is critical in controlling the activation of Tregs and maintaining immune tolerance. [Zhu et al., 2019, Cell Reports 27, 1205 1220.]

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  Q9BVI4

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human NOC4L protein was used as the immunogen for the NOC4L antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  PCRP-NOC4L-1B2

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the NOC4L antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This NOC4L antibody is available for research use only.

• Storage Conditions:

  Aliquot the NOC4L antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the NOC4L antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  FACS staining of PFA-fixed human HeLa cells using NOC4L antibody (blue, clone PCRP-NOC4L-1B2) and isotype control (red) .