MXI1 Antibody / Max-interacting protein 1 / BHLHC11

• Description:

  It is now well established that Myc regulation of cell proliferation and differentiation involves a family of related transcription factors. One such factor, Max, is an obligate heterodimeric partner for Myc and can also form heterodimers with at least four related proteins designated Mad 1, Mxi1 (also designated Mad 2), Mad 3 and Mad 4. Like Mad 1 and Mxi1, association of Mad 3 and Mad 4 with Max results in transcriptional repression. Both Myc and the Mad proteins have short half-lives and their synthesis is tightly regulated, while Max expression is constitutive and relatively stable. Two related mammalian cDNAs have been identified and shown to encode Mad- binding proteins. Both possess sequence homology with the yeast transcription repressor Sin3 including four conserved paired amphipathic helix (PAH) domains. mSin3A and mSin3B specifically interact with the Mad proteins via their second paired amphipathic helix domain (PAH2) . It has been suggested that Mad-Max heterodimers repress transcription by tethering mSin3 to DNA as corepressors.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL

• UniProt:

  P50539

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human MXI1 protein was used as the immunogen for the MXI1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  PCRP-MXI1-1A3

• Applications:

  FACS, IF, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the MXI1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MXI1 antibody is available for research use only.

• Storage Conditions:

  Aliquot the MXI1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MXI1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, cytoplasm

• Image Legend:

  Immunofluorescent staining of PFA-fixed human HeLa cells using MXI1 antibody (green, clone PCRP-MXI1-1A3) and phalloidin (red) .