E4F1 Antibody

• Description :

  E4F1 (E4F transcription factor 1), also known as E4F, is a 784 amino acid protein that localizes to both the nucleus and the cytoplasm and contains 9 C2H2-type zinc fingers. Expressed ubiquitously in adult and fetal tissues, E4F1 exists as a homodimer that binds DNA and is thought to act as a transcriptional repressor and may also play a role in cell survival and growth via cell cycle control.

• Specifications :

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt :

  Q66K89

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant full-length human E4F1 protein was used as the immunogen for the E4F1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1

• Clone :

  PCRP-E4F1-2D1

• Applications :

  FACS, IF

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the E4F1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This E4F1 antibody is available for research use only.

• Storage Conditions :

  Aliquot the E4F1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the E4F1 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Location :

  Nucleus, cytoplasm

• Image Legend :

  Immunofluorescent staining of PFA-fixed MCF7 cells using E4F1 antibody (green, clone PCRP-E4F1-2D1) and phalloidin (red) .