Daxx Antibody

• Description:

  Daxx interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, it functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  Q9UER7

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human DAXX protein was used as the immunogen for the Daxx antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  PCRP-DAXX-8B7

• Applications:

  FACS, IF

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Daxx antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Daxx antibody is available for research use only.

• Storage Conditions:

  Aliquot the Daxx antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Daxx antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, Cytoplasm

• Image Legend:

  Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using Daxx antibody (clone PCRP-DAXX-8B7) . These results demonstrate the foremost specificity of the PCRP-DAXX-8B7 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.