APOB Antibody / Apolipoprotein B

• Description:

  Post-transcriptional editing of apolipoprotein B (apoB) mRNA is regulated by APOBEC1 (also designated human (or rat) small intestinal apolipoprotein B mRNA editing protein, HEPR or REPR) in hepatic cells to achieve a steady state proportion of edited and unedited RNA molecules. Two forms of apoB are known to circulate in the plasma of mammals. ApoB-100 is a protein primarily synthesized in the liver as a structural component of very low density lipoprotein particles. A truncated form of apoB-100, apoB-48, is synthesized in the small intestine and contains the amino-terminal 2,152 amino acids of the larger protein. This organ-specific partitioning of apoB production is the result of RNA editing of a common apoB gene.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P04114

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portin of amino acids 592-689 from the human protein was used as the immunogen for the Apolipoprotein B antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b + 2IgGc κ

• Clone:

  APOB/4335

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the Apolipoprotein B antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This Apolipoprotein B antibody is available for research use only.

• Storage Conditions:

  Aliquot the Apolipoprotein B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Apolipoprotein B antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, Secreted

• Image Legend:

  IHC staining of FFPE human liver tissue with Apolipoprotein B antibody (clone APOB/4335) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.