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Recombinant ErbB2 Antibody / HER2

CAT: 0800-V9132SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9132SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 185kDa, which is identified as c-erbB-2/HER-2/neu. Its epitope is localized in the extracellular domain. C-erbB-2/HER-2 is a member of the EGFR family. This MAb is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. c-erbB-2/HER-2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.
CAS Number
9000-83-3
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
IDP04626
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant extracellular domain of human c-erbB2/HER2 was used as the immunogen for the recombinant ErbB2 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
ERBB2/4376R
Type
Recombinant
Applications
FACS, IF, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant ErbB2 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant ErbB2 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant ErbB2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant ErbB2 antibody should be determined by the researcher.
Location
Cell Surface
Image Legend
IHC staining of FFPE human breast carcinoma tissue with recombinant ErbB2 antibody (clone ERBB2/4376R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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