Anti-SUMO-1 Antibody

• Description:

  This mAb is specific to SUMO-1 and shows no cross-reaction with either SUMO2 or SUMO3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO1, SUMO2 and SUMO3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized is precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2, and E3 cascade enzymes for conjugation. However, SUMO and ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO1 protein localizes to the nuclear membrane.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P63165

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for this anti-SUMO-1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM571

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the anti-SUMO-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This anti-SUMO-1 antibody is available for research use only.

• Storage Conditions:

  Store the anti-SUMO-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear with some cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with anti-SUMO-1 antibody (clone SPM571) .