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Complement 4d Antibody / C4d

CAT: 0800-V9091-20UGSize: 20 µgDry Ice: NoHazardous: No
CAT#:0800-V9091-20UGSize:20 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d. C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1:200-1:400 for 30 min at RT
UniProt
P0C0L4, P0C0L5
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human C4d protein was used as the immunogen for this Complement 4d antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM545
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Complement 4d antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Complement 4d antibody is available for research use only.
Storage Conditions
Store the Complement 4d antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Intracytoplasmic vacuoles of endothelial cells, secreted
Image Legend
IHC testing of FFPE human kidney transplant with Complement 4d antibody (SPM545) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

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