Mucin-5AC Antibody

• Description:

  This mAb recognizes the peptide core of gastric mucin M1 (>1,000kDa) (recently identified as Mucin 5AC) . Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P98088

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  An M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le (a-b) patient was used as the immunogen for this Mucin-5AC antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM297

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the Mucin-5AC antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Mucin-5ACantibody is available for research use only.

• Storage Conditions:

  Store the Mucin-5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Mucin-5AC antibody (SPM297) .