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Fibronectin Antibody

CAT: 0800-V9020-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9020-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibronectin is a dimeric glycoprotein of 440kDa which is present in cells, extracellular matrix, and blood. It possesses at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectin is involved in cell adhesion, tissue organization, and wound healing. This mAb is directed against the peptide core and reacts with both the plasma and cellular forms of fibronectin.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P02751
Host
Mouse
Reactivity
Human
Immunogen
Purified human fibronectin was used as the immunogen for this Fibronectin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM539
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fibronectin antibody is available for research use only.
Storage Conditions
Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the Fibronectin antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 minutes.
Location
Connective tissue matrix
Image Legend
IHC: Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma stained with Fibronectin antibody (SPM539) .

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