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PCNA Antibody

CAT: 0800-V8995-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V8995-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P12004
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human PCNA protein was used as the immunogen for the PCNA antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PCNA/6580
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the PCNA antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the PCNA antibody should be determined by the researcher.
Location
Predominantly nuclear, some cytoplasmic
Image Legend
IHC staining of FFPE human tonsil tissue with PCNA antibody (clone PCNA/6580) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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