TCF4 Antibody

• Description:

  Recognizes a protein of 71kDa, identified as Transcription Factor 4 (TCF4) . It is a basic helix-loop-helix transcription factor. The encoded protein recognizes an Ephrussi-box () - a motif first identified in immunoglobulin enhancers. This gene is broadly expressed and may play an important role in nervous system development. Defects in this gene are a cause of Pitt-Hopkins syndrome.

• Specifications:

  ELISA (For coating, order BSA-free format), Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P15884

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 365-671 was used as the immunogen for the TCF4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  TCF4/2252

• Applications:

  ELISA, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the TCF4 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This TCF4 antibody is available for research use only.

• Storage Conditions:

  Aliquot the TCF4 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TCF4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus, Cytoplasm

• Image Legend:

  IHC staining of FFPE human breast carcinoma tissue with TCF4 antibody (clone TCF4/2252) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.