ZHX3 Antibody

CAT: 0800-V8872-100UGSize: 100 µgDry Ice: NoHazardous: No

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CAT#:0800-V8872-100UGSize:100 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

Zinc-fingers and homeobox (ZHX) proteins are transcription factors that interact with the activation domain of the A subunit of nuclear factor-Y (NF-YA) . ZHX1-3 are ubiquitously expressed proteins expressed in various tissues. They act as transcriptional repressors and localize to the nucleus. The ZHX proteins contain two Cys2-His2-type zinc-finger motifs and five homeodomains (HDs) . These domains allow the ZHX proteins to form homodimers, but they can also form heterodimers with each other. However, this dimerization is not required for repressor activity. Hypermethylation-mediated silencing of ZHX2 is an epigenetic event involved in hepatocellular carcinoma (HCC) .

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate), Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells

UniProt

Q9H4I2

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human ZHX3 protein was used as the immunogen for the ZHX3 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-ZHX3-1D11

Applications

ELISA, IP, WB, FACS

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the ZHX3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This ZHX3 antibody is available for research use only.

Storage Conditions

Aliquot the ZHX3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the ZHX3 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

Analysis of HuProt (TM) microarray containing more than 19,000 full-length human proteins using ZHX3 antibody (clone PCRP-ZHX3-1D11) . These results demonstrate the foremost specificity of the PCRP-ZHX3-1D11 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

UniProtKB · Q9H4I2

Zinc fingers and homeoboxes protein 3

ZHX3_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: Q9H4I2
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: ZHX3
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: —
Secondary accessions: E1P5W5, F5H820, O43145, Q6NUJ7

Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

DifferentiationTranscriptionTranscription regulation

Molecular function

DNA-bindingRepressor

Domain

HomeoboxRepeatZinc-finger

Ligand

Metal-bindingZinc

Cellular component