MITF Antibody

• Description:

  MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer and is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP-dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP1 and TRP2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Ser73 (via the MAPK pathway), it associates with co-activators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In MITFdeficient mice there is a complete absence of melanocytes.

• Specifications:

  Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate), Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells, Western blot: 1-2 µg/mL

• UniProt:

  O75030

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human MITF protein was used as the immunogen for the MITF antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b

• Clone:

  PCRP-MITF-1D9

• Applications:

  IP, IF, FACS, WB

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the MITF antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This MITF antibody is available for research use only.

• Storage Conditions:

  Aliquot the MITF antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MITF antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  FACS staining of PFA-fixed human HeLa cells with MITF antibody (blue, clone PCRP-MITF-1D9) and isotype control (red) .