Recombinant CD30 Antibody

• Description:

  Recognizes a single chain glycoprotein of 105/120kDa, identified as CD30/Ki-1. CD30 is synthesized as a 90kDa precursor, which is processed in the Golgi complex into a membrane-bound phosphorylated mature 105/120kDa glycoprotein. In Hodgkin s disease, CD30/Ki-1 antigen is expressed by mononuclear-Hodgkin and multinucleated Reed-Sternberg cells. It is also expressed by the tumor cells of a majority of anaplastic large cell lymphomas as well as by a varying proportion of activated T and B cells. This MAb distinguishes large cell lymphomas derived from activated lymphoid cells from histiocytic malignancies and lymphomas derived from resting and precursor lymphoid cells or from anaplastic carcinomas. About one third of the Ki-1 positive lymphomas lack the leukocyte common antigen (CD45) .

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P28908

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Recombinant human full-length protein was used as the immunogen for the recombinant CD30 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  Ki-1/4399R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the recombinant CD30 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant CD30 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant CD30 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant CD30 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC testing of FFPE Hodgkin's lymphoma stained with recombinant CD30 antibody (clone Ki-1/4399R) . Required HIER: steam sections in pH9, 1mM EDTA for 10-20 min.