Recombinant SATB2 Antibody

• Description:

  SATB2 (Special AT-rich sequence-binding protein 2) is a DNA binding protein that specifically binds nuclear matrix attachment regions. It is involved in transcription regulation and chromatin remodeling. SATB2 expression in colorectal carcinomas (CRC) is correlated with good prognosis and in laryngeal squamous cell carcinoma it functions as a tumor suppressor wherein loss of expression is positively correlated with high tumor grade and recurrence. Moreover, SATB2, in combination with CK20, could identify almost all CRC s. Upper gastrointestinal (GI) carcinomas and pancreatic ductal carcinomas are usually negative for SATB2, and ovarian carcinomas, lung adenocarcinomas, and adenocarcinomas from other origin are rarely positive for SATB2. Additionally, SATB2 antibody can identify neuroendocrine neoplasms of colon and rectum because SATB2 is usually negative in neuroendocrine neoplasms of the GI tract, pancreas, and lung. More recently, it has been reported that SATB2 is a sensitive marker for tumors with osteoblastic differentiation.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  Q9UPW6

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 200-300 from the human protein was used as the immunogen for the recombinant SATB2 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG

• Clone:

  SATB2/4374R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the recombinant SATB2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant SATB2 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant SATB2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the recombinant SATB2 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human colon carcinoma with recombinant SATB2 antibody (clone SATB2/4374R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.