SLAMF7 Antibody / CS1

• Description:

  CS1, also known as novel Ly9, SLAMF7, 19A24 or CRACC, is a homophilic cell surface receptor. It is a member of the SLAM (signaling lymphocytic activation molecule) family of receptors expressed on natural killer (NK) cells, T cells and stimulated B cells. CS1 contains immunoreceptor tyrosine-based switch motifs in its cytoplasmic domain but, unlike other SLAM receptors, it does not recruit SAP (SLAM-associated protein) . In humans, CS1 activates NK cells through an EAT-2-mediated pathway that is SAP-independent. CS1 recruits and associates with EAT-2, a protein closely related to SAP. EAT-2 induces phosphorylation of CS1 which then, upon ligand binding, activates downstream cytotoxicity effectors PLC and PI 3K. In mice, the EAT-2 association with CS1 has an inhibitory effect on the activation of NK cells.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  Q9NQ25

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 249-335 from the human protein was used as the immunogen for the SLAMF7 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SLAMF7/3649

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the SLAMF7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This SLAMF7 antibody is available for research use only.

• Storage Conditions:

  Store the SLAMF7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the SLAMF7 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasm, cell surface

• Image Legend:

  IHC staining of FFPE human spleen with SLAMF7 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.