PSMD4 Antibody

• Description:

  In eukaryotic cells, selective breakdown of cellular proteins is ensured by two distinct pathways. First, appropriate proteins are tagged for degradation by ubiquitination. Second, these multiubiquitinated proteins are degraded by the highly selective 26S Proteasome protein-destroying machinery. At specific stages of development, embryo- and tissue-specific components of the 26S Proteasome are formed, which are termed Rpn10a through Rpn10e, or alternatively pUb-R2 through pUb-R5. All members of this family can be generated by a single Rpn10 gene by developmentally regulated alternative splicing. The pUb-R2 subunit, originally identified as S5a (also designated antisecretory factor and multiubiquitin chain binding protein) is ubiquitously expressed and may perform proteolysis constitutively in a wide variety of cells. pUb-R4 and pUb-R5 may have embryo- or tissue-specific expression and may play specialized roles in early embryonic development.

• Specifications:

  Western blot: 1-2 µg/mL

• UniProt:

  P55036

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human PSMD4 protein was used as the immunogen for the PSMD4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CPTC-PSMD4-3

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the PSMD4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PSMD4 antibody is available for research use only.

• Storage Conditions:

  Store the PSMD4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the PSMD4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nucleus and cytoplasm

• Image Legend:

  Western blot testing of human liver lysate with PSMD4 antibody. Predicted molecular weight ~41 kDa, routinely observed at ~50 kDa.