CD40 Antibody

• Description:

  Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. FGK4.5 can be used for blocking of CD40/CD154 interaction and forin vitroandin vivoactivation of CD40 expressing APCs. This MAb is shown to indirectly activate natural killer (NK) cells, producing significant antitumor and anti-metastatic effects. Effective in boosting immune responses against infectious agents and can potentially be used to treat chronic autoimmune inflammatory processes.

• Specifications:

  Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-3 µg/mL

• UniProt:

  P27512

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  Purified recombinant Mouse CD40 fusion protein was used as the immunogen for the CD40 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  FGK45.5

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD40 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD40 antibody is available for research use only.

• Storage Conditions:

  Store the CD40 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD40 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4