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Clostridium difficile Toxin A Antibody

CAT: 0800-V8285-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8285-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb reacts with C. difficile Toxin A, but not with Cholera subunit a, Cholera toxin, Pseudomonas aeruginosa exotoxin A, H-LT, P-LT. Clostridium difficile is a major nosocomial pathogen that causes antibiotic- associated colitis. Clostridium difficile mediates inflammatory diarrhea by releasing two large protein enterotoxins (Toxin A and Toxin B) that are able to disrupt intestinal epithelial cells via their transferase activity and ability to monoglucosylate members of the Rho family. Clostridium difficile Toxin A is a toxin that is composed of 39 repeats that are responsible for binding to intestinal epithelial cell surface carbohydrates. Clostridium difficile Toxin A causes significant apoptosis of colonocytes which contributes to the formation of ulcers and pseudo-membranes in a pathway that involves p38-dependent activation of p53 and induction of p21, leading to cytochrome c release and caspase-3 activation through Bak activation.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Immunofluorescence: 1-2 µg/mL
UniProt
P16154
Host
Mouse
Reactivity
Clostridium difficile
Immunogen
C. difficile Toxin A was used as the immunogen for the Clostridium difficile Toxin A antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
253/422
Applications
ELISA, IF
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Clostridium difficile Toxin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Clostridium difficile Toxin A antibody is available for research use only.
Storage Conditions
Store the Clostridium difficile Toxin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Clostridium difficile Toxin A antibody should be determined by the researcher.

UniProtKB · P16154

Toxin A

TCDA_CLODI · Clostridioides difficile

View on UniProt ↗
Primary accession
P16154
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
tcdA
Protein existence
1: Evidence at protein level
Organism
Clostridioides difficile (Peptoclostridium difficile)
Taxonomy ID
1496
Alternative names
—
EC number
3.4.22.-
Processing
—
Secondary accessions
M4NKU2
Protein keywords

Technical term

3D-structure

PTM

Autocatalytic cleavage

Molecular function

EnterotoxinGlycosyltransferaseHydrolaseProteaseThiol proteaseToxinTransferase

Cellular component

Host cell membraneHost cytoplasmHost endosomeHost membraneMembraneSecreted

Ligand

Lipid-bindingMagnesiumManganeseMetal-bindingZinc

Domain

Repeat

Biological process

Virulence
Product Datasheet
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