HIF1 alpha Antibody

HIF1 (hypoxia-inducible factor 1), a heterodimeric transcription factor complex central to cellular response to hypoxia, consists of two subunits (HIF-1 alpha and HIF-1 beta) which are basic helix-loop-helix proteins of the PAS (Per, ARNT, Sim) family. Expression of HIF-1 alpha protein is regulated by cellular oxygen level alterations as well as in oxygen-independent manner via different cytokines (through the PI3K-AKT-mTOR pathway), growth factors, oncogenic activation, or loss of tumor suppressor function etc. In normoxic cells, HIF-1 alpha is proline hydroxylated leading to a conformational change that promotes its binding to the VLH (von Hippel Lindau) protein E3 ligase complex; ubiquitination and followed by rapid proteasomal degradation. Hypoxia as well as chemical hydroxylase inhibitors (desferrioxamine, cobalt etc.) inhibit HIF-1 alpha degradation and lead to its accumulation in the cells, whereas, contrastingly, HIF-1 beta/ARNT (AhR nuclear translocator) remains stable under both conditions. Besides their critical role in hypoxic response, HIF1s regulates the transcription of genes responsible for angiogenesis, erythropoiesis/iron-metabolism, glucose metabolism, cell proliferation/survival, adipogenesis, carotid body formation, B lymphocyte development and immune reactions.

9007-83-4

ELISA (order Ab without BSA for coating), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

Q16665

Human

A portion of amino acids 239-530 from the human protein was used as the immunogen for the HIF1 alpha antibody.

Ha111a

ELISA, WB, IHC-P

Protein G affinity chromatography

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the HIF1 alpha antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This HIF1 alpha antibody is available for research use only.

Store the HIF1 alpha antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the HIF1 alpha antibody should be determined by the researcher.

Nuclear and cytoplasmic