Recombinant SOX2 Antibody

• Description:

  SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression. Reportedly, SOX2 is associated with aggressive phenotypes of breast, head and neck, gastric, colorectal, bladder, and small cell lung cancers. However, SOX2 is expressed in a high percentage of lung squamous cell carcinomas and has been shown to be an independent favorable prognostic marker.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL (not suitable for frozen tissues)

• UniProt:

  P48431

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  Amino acids 176-305 were used as the immunogen for the recombinant SOX2 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  RSOX2/1791

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the recombinant SOX2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant SOX2 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant SOX2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant SOX2 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human cervix with recombinant SOX2 antibody (clone rSOX2/1791) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.