BMI1 Antibody

• Description:

  The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

• Specifications:

  Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P35226

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  A recombinant human partial protein (amino acids 142-326) was used as the immunogen for this BMI1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BMI1/2823

• Applications:

  WB, IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This BMI1 antibody is available for research use only.

• Storage Conditions:

  Store the BMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the BMI1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  Western blot testing of mouse NIH3T3 cell lysate with BMI1 antibody. Predicted molecular weight: 37-43 kDa.