PPP3CA Antibody / Calcineurin

• Description:

  Calcineurin is an enzyme that dephosphorylates serine and threonine residues in proteins. It is a heterodimer of a 59kDa catalytic A subunit and a 19kDa regulatory B subunit that is activated by the binding of calcium ions and calmodulin. Calcineurin is expressed in many tissues, but its levels are highest in the brain, where it may play a role in learning and memory. It has many substrates, including NFAT, a transcription factor that is activated by dephosphorylation. Complexes of the immuno-suppressants cyclosporine and FK506 with immunophilin proteins such as cyclophilin and FKBP12 are potent and specific inhibitors of Calcineurin activity. Alterations in Calcineurin activity are suspected to play a role in cardiac hypertrophy and graft versus host disease in organ transplantation.

• Specifications:

  Western blot: 1-2 µg/mL

• UniProt:

  Q08209

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant full-length Calcineurin A protein was used as the immunogen for the PPP3CA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BF1.1

• Applications:

  WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the PPP3CA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PPP3CA antibody is available for research use only.

• Storage Conditions:

  Store the PPP3CA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the PPP3CA antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free PPP3CA antibody (clone BF1.1) as confirmation of integrity and purity.