PCNA Antibody

• Description:

  Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P12004

• Host:

  Mouse

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  PC5

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PCNA antibody is available for research use only.

• Storage Conditions:

  Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PCNA antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Predominantly nuclear, some cytoplasmic

• Image Legend:

  IHC staining of FFPE human tonsil with PCNA antibody (clone PC5) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.