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PCNA Antibody

CAT: 0800-V7998-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7998-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA) . It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P12004
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
A rat PCNA/protein A fusion protein was used at the immunogen for this PCNA antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
PC5
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the PCNA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This PCNA antibody is available for research use only.
Storage Conditions
Store the PCNA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the PCNA antibody should be determined by the researcher.
Location
Predominantly nuclear, some cytoplasmic
Image Legend
IHC staining of FFPE human tonsil with PCNA antibody (clone PC5) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Product Datasheet
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