CD95 Antibody / Fas

CAT: 0800-V7883-20UGSize: 20 µgDry Ice: NoHazardous: No

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CAT#:0800-V7883-20UGSize:20 µg

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

This MAb specifically recognizes CD95, also known as Fas, a transmembrane glycoprotein with a MW of 40-45kDa, containing 8kDa of N-glycoside-linked polysaccharide. It is a receptor for TNFSF6/FASLG, a member of the nerve growth factor receptor/tumor necrosis factor superfamily, mediating receptor-triggered apoptosis. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation, which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. The secreted isoforms 2 to 6 block apoptosis (in vitro) . CD95 antigen is expressed on the surface of various cell types, preferentially on the CD45RAlow CD45ROhigh subset of memory T lymphocytes.

CAS Number

9007-83-4

Specifications

ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

UniProt

P25445

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein (amino acids 26-96) was used as the immunogen for the CD95 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FAS/3588

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the CD95 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD95 antibody is available for research use only.

Storage Conditions

Store the CD95 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the CD95 antibody should be determined by the researcher.

Location

Cytoplasm, membrane

Image Legend

IHC staining of FFPE human tonsil with CD95 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · P25445

Tumor necrosis factor receptor superfamily member 6

TNR6_HUMAN · Homo sapiens

View on UniProt ↗

Primary accession: P25445
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: FAS
Protein existence: 1: Evidence at protein level
Organism: Homo sapiens (Human)
Taxonomy ID: 9606
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: A9UJX4, B6VNV4, Q14292, Q14293, Q14294, Q14295, Q16652, Q5T9P1, Q5T9P2, Q5T9P3, Q6SSE9

Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Apoptosis

Molecular function

Calmodulin-bindingReceptor

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variant

PTM

Disulfide bondGlycoproteinLipoproteinPalmitatePhosphoprotein

Domain

RepeatSignalTransmembraneTransmembrane helix

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Alternative Products

Catalog	Name
GTR17856933	CD95 Antibody