HLA-DRB1 Antibody

• Description:

  This MAb reacts with a 28kDa chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells

• UniProt:

  P01911

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human REH cells (a non-T, non-B leukemia cell line) were used as the immunogen for the HLA-DRB1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  Bra30

• Applications:

  FACS

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the HLA-DRB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This HLA-DRB1 antibody is available for research use only.

• Storage Conditions:

  Store the HLA-DRB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the HLA-DRB1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4