Annexin A1 Antibody

• Description:

  The ANXA1 gene belongs to the annexin family, and contains 4 annexin repeats. A pair of annexin repeats may form one binding site for calcium and a phospholipid. ANXA1 promotes membrane fusion and is involved in exocytosis. The gene for ANXA1 is upregulated in hairy cell leukemia (HCL), and its protein expression is specific for HCL. Detection of ANXA1 provides a simple, highly sensitive and specific assay for diagnosing HCL. Annexin A1 has also been found to be protective against DNA damage induced by heat in breast cancer cells, suggesting it is involved in tumor suppressive and protective activities, and also is associated with treatment resistance.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL

• UniProt:

  P04083

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human Annexin A1 protein was used as the immunogen for the Annexin A1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  ANXA1/3566

• Applications:

  FACS, IF, IHC-P, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Annexin A1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Annexin A1 antibody is available for research use only.

• Storage Conditions:

  Store the Annexin A1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Annexin A1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic, nuclear

• Image Legend:

  Immunofluorescent staining of PFA-fixed human HeLa cells with Annexin A1 antibody (green, clone ANXA1/3566) and Reddot nuclear stain (red) .