Products for Research Use Only
RegisterLogin

Nestin Antibody

CAT: 0800-V7665-20UGSize: 20 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0800-V7665-20UGSize:20 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
Nestin is a major intermediate filament (IF) protein of embryonic central nervous system progenitor cells. It is also a component of the dynamic IF network during muscle development, where it polymerizes with Desmin and Vimentin. Nestin co-assembles with Vimentin or -internexin and forms heterodimer coiled-coil molecules which then further assemble into 10 nml IFs. Deletion of the IF consensus rod domain in nestin alters nestin localization in CNS precursor cells and radial glial cells in vivo. Nestin is a marker for neuroepithelial stem cells, glioma cells and tumor endothelial cells during rapid growth. During axon elongation of differentiation neurons, nestin localizes to the growth cones and may play a role in growth cone guidance. In the rat adrenal gland, nestin is expressed by the zona fasciculata and the zona reticularis. Nestin is also expressed by dermatomal cells and by myoblasts during the earliest stages of myogenesis.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P48681
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 698-844) was used as the immunogen for the Nestin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NES/2911
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Nestin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nestin antibody is available for research use only.
Storage Conditions
Store the Nestin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Nestin antibody should be determined by the researcher.
Location
Cytoplasm, membrane
Image Legend
IHC staining of FFPE human kidney with Nestin antibody (clone NES/2911) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

UniProtKB · P48681

Nestin

NEST_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P48681
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
NES
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
O00552, Q3LIF5, Q5SYZ6
Protein keywords

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Domain

Coiled coil

Molecular function

Developmental protein

Cellular component

Intermediate filament

Biological process

Neurogenesis

Technical term

Proteomics identificationReference proteome

Related Products

CatalogName
RQ8919NES Antibody / Nestin
RQ7746Nesprin 3 Antibody / SYNE3
FY12952SYNE3 Antibody / Nesprin 3
FY13047Nesprin 3 Antibody / SYNE3
RQ4624NONO Antibody / p54nrb Antibody
F53954-0.05MLAntibody
F53954-0.2MLAntibody
F53952-0.2MLAntibody
F53952-0.05MLAntibody
V8674-20UGInhibin alpha Antibody / INHA antibody

Alternative Products

CatalogName
70R-10645Nestin antibody
ABD7754Nestin Antibody

Popular Products