Recombinant Wilms Tumor 1 Antibody

• Description:

  Wilms Tumor is a transcription factor that plays an important role in cellular development and cell survival. Regulates the expression of numerous target genes, including EPO. Plays an essential role for development of the urogenital system. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'. It has a tumor suppressor as well as an oncogenic role in tumor formation. Function may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. [UniProt]

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P19544

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human protein was used as the immunogen for the recombinant Wilms Tumor 1 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG1 k

• Clone:

  MMWT1-1

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the recombinant Wilms Tumor 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Wilms Tumor 1 antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Wilms Tumor 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9000-83-3

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  IHC testing of FFPE human mesothelioma with recombinant Wilms Tumor 1 antibody (clone MMWT1-1) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.