TIGIT Antibody

CAT: 0800-V7584BTNSize: 500 µLDry Ice: NoHazardous: No
CAT#:0800-V7584BTNSize:500 µL
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is a immuno-oncology target for therapy.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT
UniProt
Q495A1
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 22-141 from the human protein was used as the immunogen for the TIGIT antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
TIGIT/3106
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Biotin Conjugate
Buffer
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the TIGIT antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Limitations
This TIGIT antibody is available for research use only.
Storage Conditions
Store the TIGIT antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .
Formulation
0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the TIGIT antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human prostate carcinoma with biotinylated TIGIT antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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