TIGIT Antibody

• Description:

  TIGIT is a checkpoint inhibitor which binds with high affinity to the poliovirus receptor (PVR), causing increased IL10 secretion, decreased IL12B secretion. TIGIT binding to PVR also causes the suppression of T cell activation by promoting the generation of mature immuno-regulatory dendritic cells. It is expressed at low levels on natural killer (NK) cells, as well as peripheral memory and regulatory CD4+ T cells. At the protein level, it is upregulated following the activation of these cells. Functionally, TIGIT is similar to CTLA4. The ligands for TIGIT include CD155 (signal abrogation) and CD226 (signal stimulation) . It has been demonstrated to be upregulated on T cells in many cancers and is a immuno-oncology target for therapy.

• Specifications:

  Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

• UniProt:

  Q495A1

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 22-141 from the human protein was used as the immunogen for the TIGIT antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  TIGIT/3106

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Biotin Conjugate

• Buffer:

  0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the TIGIT antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

• Limitations:

  This TIGIT antibody is available for research use only.

• Storage Conditions:

  Store the TIGIT antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

• Formulation:

  0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the TIGIT antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC staining of FFPE human prostate carcinoma with biotinylated TIGIT antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.