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VISTA Antibody / GI24

CAT: 0800-V7579-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V7579-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
VISTA / GI24 is a transmembrane protein expressed in bone, on embryonic stem cells (ESCs), and on tumor cell surfaces. On ESC's, Gi24 appears to positively interact with BMP-4, potentiating BMP signaling and the transition from an undifferentiated to a differentiated state. On tumor cells, Gi24 both promotes MT1-MMP expression and activity and serves as a substrate for MT1-MMP. This increases the potential for cell motility. Mature human Gi24 contains a 162aa extracellular region with one V-type Ig-like domain and a 96aa cytoplasmic domain. Human Gi24 undergoes proteolytic cleavage by MT1-MMP, generating a soluble 30kDa extracellular fragment plus a 25-30kDa membrane-bound fragment. VISTA is a negative checkpoint regulator and is expressed on myeloid cells, T-cells and human TILs (tumor infiltrating lymphocytes) on MDSCs (myeloid-derived suppressor cells) in the TME (tumor microenvironment) . It is very likely both a ligand and receptor and is a promising target for cancer immunotherapy.
CAS Number
9007-83-4
Specifications
ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q9H7M9
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human VISTA protein was used as the immunogen for the VISTA antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
VISTA/3006
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the VISTA antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This VISTA antibody is available for research use only.
Storage Conditions
Store the VISTA antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell membrane, cytoplasm
Image Legend
IHC staining of FFPE human placenta with VISTA antibody (clone VISTA/3006) . HIER: boil tissue sections in pH 9, 10mM Tris with 1mM EDTA, for 10-20 min and allow to cool before testing.

UniProtKB · Q9H7M9

V-type immunoglobulin domain-containing suppressor of T-cell activation

VISTA_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9H7M9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
VSIR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1L0X9, A4ZYV1, A8MVH5, Q6UXF3, Q8WUG3, Q8WYZ8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

Disulfide bondGlycoproteinPhosphoprotein

Domain

Immunoglobulin domainSignalTransmembraneTransmembrane helix

Molecular function

Receptor

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Alternative Products

CatalogName
GTR18574654VISTA Antibody / GI24
GTR18225379VSIR/GI24/VISTA Antibody
AN200229PVISTA/GI24/B7-H5 Monoclonal Antibody
36-3148-100Anti-VISTA / GI24 (Negative Regulator of Immune Response) Monoclonal Antibody (Clone: VISTA/3006)
36-3147-20Anti-VISTA / GI24 (Negative Regulator of Immune Response) Monoclonal Antibody (Clone: VISTA/2865)
36-3146-20Anti-VISTA / GI24 (Negative Regulator of Immune Response) Monoclonal Antibody (Clone: VISTA/2864)
AMM22005VGI24 (VISTA (C10ORF54) ) Mouse Monoclonal Antibody [Clone ID: UMLBI272]
AMM22004VGI24 (VISTA (C10ORF54) ) Mouse Monoclonal Antibody [Clone ID: UMLBI271]
E45M22004V-2GI24 (VISTA (C10ORF54) ) Mouse Monoclonal Antibody [Clone U mLBI271]
E45M22005V-2GI24 (VISTA (C10ORF54) ) Mouse Monoclonal Antibody [Clone U mLBI272]

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