Sarcomeric Alpha Actinin Antibody / ACTN2

• Description:

  Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In non-muscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific, alpha actinin isoform that is expressed in both skeletal and cardiac muscles. Several transcript variants encoding different isoforms have been found for this gene.

• Specifications:

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P35609

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids 557-692 from the human protein were used as the immunogen for this Sarcomeric Alpha Actinin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  ACTN2/3293

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Sarcomeric Alpha Actinin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Sarcomeric Alpha Actinin antibody is available for research use only.

• Storage Conditions:

  Store the Sarcomeric Alpha Actinin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of human skeletal muscle lysate with Sarcomeric Alpha Actinin antibody (clone ACTN2/3293) . Predicted molecular weigth ~103 kDa.