CD47 Antibody

• Description:

  This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

• Specifications:

  Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

• UniProt:

  Q08722

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 18-135 from the human protein was used as the immunogen for the CD47 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2c κ

• Clone:

  CD47/2937

• Applications:

  WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Biotin Conjugate

• Buffer:

  0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD47 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

• Limitations:

  This CD47 antibody is available for research use only.

• Storage Conditions:

  Store the CD47 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

• Formulation:

  0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD47 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  IHC staining of FFPE human prostate carcinoma with biotinylated CD47 antibody (clone CD47/2937) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.