Beta Catenin Antibody

• Description :

  Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P35222

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A full length human recombinant protein was used as the immunogen for the Beta Catenin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  CTNNB1/2099

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This Beta Catenin antibody is available for research use only.

• Storage Conditions :

  Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic, cell junctions

• Image Legend :

  IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone CTNNB1/2099) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.