CD163 Antibody

CAT: 0800-V7470IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
CAT#:0800-V7470IHC-7MLSize:7 mL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a protein of 140kDa, identified as CD163. It has been identified as an acute phase-regulated transmembrane protein whose function is to mediate the endocytosis of haptoglobin-hemoglobin complexes. This receptor is expressed on the surface of monocytes with low expression and on tissue macrophages, histiocytes with high expression. Staining with anti-CD163 has been helpful to distinguish synovial macrophages from synovial intimal fibroblasts in rheumatoid arthritis, where its specificity for macrophages was found to be superior to that of anti-CD68. Increased levels of CD163 were also detected in patients with microbial infections and myelomonocytic leukemias. Anti-CD163 is of considerable value for selective identification of monocytes and macrophages at a certain stage of differentiation and is suitable for diagnosing myelomonocytic or monocytic leukaemia and neoplasms of true histiocytic origin. CD163 is positive in skin (histiocytes), gut, Kupffer cells, a few alveolar macrophages, macrophages in the placenta, and in macrophages in inflamed tissues including tumor tissue.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
Q86VB7
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein corresponding to amino acids 43-196 was used as the immunogen for the CD163 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
M130/2164
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the CD163 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD163 antibody is available for research use only.
Storage Conditions
Store the CD163 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Cell membrane, cytoplasm
Image Legend
IHC testing of FFPE human lymph node with CD163 antibody (clone M130/2164) . HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 for 10-20 min followed by cooling at RT for 20 min.

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