MMP3 Antibody

• Description:

  The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP3, MMP10 and MMP11 (also designated stromelysin-1, 2 and 3, respectively) activate procollagenase. MMP3 activation of procollagenase can occur via two pathways. Direct activation by MMP3 is slow and activation by MMP3 in conjunction with tissue or plasma proteinases is rapid. MMP10 is expressed in small intestine, and at lower levels in lung and heart. MMP11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Western blot: 1-2 µg/mL

• UniProt:

  P08254

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 236-363 was used as the immunogen for the MMP3 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MMP3/2655

• Applications:

  ELISA, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the MMP3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MMP3 antibody is available for research use only.

• Storage Conditions:

  Store the MMP3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, secreted

• Image Legend:

  Western blot testing of human Raji, HEK293, and HT-29 cell lysate with MMP3 antibody. Predicted molecular weight ~54 kDa.