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Recombinant MCM7 Antibody

CAT: 0800-V7360IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7360IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProt]
CAS Number
9000-83-3
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
P33993
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids 195-319 from the human protein were used as the immunogen for the recombinant MCM7 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
MMCC7-1R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the recombinant MCM7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant MCM7 antibody is available for research use only.
Storage Conditions
Store the recombinant MCM7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC testing of FFPE human lymph node with recombinant MCM7 antibody (clone MMCC7-1R) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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