PS2 Antibody / TFF1

• Description :

  It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. It is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

• Specifications :

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt :

  P04155

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant human protein was used as the immunogen for this pS2 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  TFF1/2133

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution :

  Store the pS2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This pS2 antibody is available for research use only.

• Storage Conditions :

  Store the pS2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic, secreted

• Image Legend :

  IHC staining of FFPE human breast carcinoma with pS2 antibody (clone TFF1/2133) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.