BMI1 Antibody

• Description :

  The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.

• Specifications :

  Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P35226

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A portion of amino acids 142-326 from the human protein was used as the immunogen for the BMI1 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  BMI1/2689

• Applications :

  WB, IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This BMI1 antibody is available for research use only.

• Storage Conditions :

  Store the BMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• CAS Number :

  9007-83-4

• Location :

  Nuclear

• Image Legend :

  IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone BMI1/2689) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.