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BMI1 Antibody

CAT: 0800-V7349-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7349-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The B cell-specific moloney murine leukemia virus integration site 1 (Bmi-1) is a transcriptional receptor of the polycomb gene family involved in several cellular processes including cell-cycle regulation, apoptosis, and maintenance of adult and neoplastic stem cells by providing self-renewal capacity. Further, Bmi-1 expression has been associated with malignant transformation, tumor progression, metastatic disease, and poor prognosis in human malignancies.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P35226
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 142-326 from the human protein was used as the immunogen for the BMI1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
BMI1/2689
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the BMI1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BMI1 antibody is available for research use only.
Storage Conditions
Store the BMI1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
IHC testing of FFPE human colon carcinoma with BMI1 antibody (clone BMI1/2689) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

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