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BOB1 Antibody

CAT: 0800-V7324IHC-7MLSize: 7 mLDry Ice: NoHazardous: No
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CAT#:0800-V7324IHC-7MLSize:7 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
BOB1, also called POU2AF1, expression in a variety of established B-cell lines, representing different stages of B-cell development, has suggested a constitutive, B-cell-specific expression pattern. LP cells in nodular lymphocyte predominant Hodgkin lymphoma, because they are germinal center-derived, are consistently immuno-positive for BOB1. Conversely, only some cases of classical Hodgkin lymphoma show BOB1 immuno-reactivity within the Hodgkin and Reed-Sternberg cells. Expression of BOB1 has been reported in follicular center cell lymphoma, diffuse large B-cell lymphoma and some cases of acute myeloid leukemia. B-CLL, marginal zone lymphoma, and mantle cell lymphoma may show weak to moderate immunoreactivity.
CAS Number
9007-83-4
Specifications
The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
UniProt
Q16633
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 148-255 from the human protein was used as the immunogen for the BOB1 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
BOB1/2421
Applications
IHC-P
Purity
Protein G affinity
Format
Purified
Buffer
Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*
Reconstitution
Store the BOB1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This BOB1 antibody is available for research use only.
Storage Conditions
Store the BOB1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*
Location
Nuclear
Image Legend
IHC testing of FFPE human spleen with BOB1 antibody (clone BOB1/2421) . HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

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