MAML2 Antibody

• Description :

  Mastermind-like 2, also known as MAM2, MAM3 or MLLMAML2, is a nuclear speckle protein that acts as a transcriptional co-activator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML2 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, it interacts with the ankyrin repeats of the Notch proteins (1-4) . This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML2 is essential for proper Notch binding, the C-terminal domain of MAML2 is essential for transcriptional activation. A chromosomal aberration involving the gene is implicated in mucoepidermoid carcinomas, clear cell hidradenomas and benign Warthin tumors.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A human recombinant protein was used as the immunogen for this MAML2 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  MMLP2-1

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the MAML2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This MAML2 antibody is available for research use only.

• Storage Conditions :

  Store the MAML2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MAML2 antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cytoplasmic

• Image Legend :

  IHC testing of FFPE human bladder carcinoma with MAML2 antibody (clone MMLP2-1) . Staining of FFPE tissue requires boiling sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min followed by cooling at RT for 20 min.