CFTR Antibody

• Description :

  Cystic fibrosis transmembrane conductance regulator (CFTR) is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (MDR1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP) -activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt :

  P13569

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Human recombinant protein from the C-terminal region (within amino acids 1350-1480) was used as the immunogen for this CFTR antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2a κ

• Clone :

  CFTR1-1

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1X PBS, pH 7.4

• Reconstitution :

  Store the CFTR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CFTR antibody is available for research use only.

• Storage Conditions :

  Store the CFTR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CFTR antibody to be titered up or down for optimal performance.

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  IHC testing of FFPE human pancreas with CFTR antibody (clone CFTR1-1) . Staining of FFPE tissue is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH9 for 10-20 min followed by cooling at RT for 20 min.